Figure 4

Elevated IOP increases glial markers in retina and optic nerve. Confocal images from saline- and microbead-injected SM eyes showing immunolabeling (red) for GFAP or Iba1 in the retina (a,e) and in the optic nerve (c,g). CTB (green) in RGC somas and axons is shown. NFL: nerve fiber layer; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer. Label was quantified in region bounded by yellow dotted lines in retinal images. Bar graphs show percent area of image that was immunolabeled for GFAP in retina and optic nerve (b,d) and Iba1 in the retina and optic nerve (f,h). *p = 0.01 (b), p = 0.00003 (d), p = 0.0003 (f), p = 0.0006 (h). n indicated in bars. Data are expressed as mean ± SEM. Statistical comparisons were made using two-sided t-tests.