Figure 6

Pairwise comparison by OPLS-DA and volcano plot of spinal cord lipidomes of SOD1-G93A 120d and WT 120d groups. (A) Multivariate statistical analysis using orthogonal partial least squares–discriminate analysis (OPLS-DA); (B) Feature importance of the top 20 lipid species altered according to the OPLS-DA analysis; (C) Volcano plot is represented by the log₂ (fold change) of SOD1-G93A 120d/WT 120d groups plotted against the –log₁₀ (p). Statistical significance was evaluated by t-test (p < 0.05; FDR-adjusted) and the fold change was set to >1.5. The significantly altered lipid molecular species are colored according to lipid subclasses. List of altered cardiolipins is sorted by log₂ (fold change).