Figure 1 | Scientific Reports

Figure 1

From: Functional expression of CLIFAHDD and IHPRF pathogenic variants of the NALCN channel in neuronal cells reveals both gain- and loss-of-function properties

Figure 1

The NG108-15 cell line endogeneously expresses Unc79 and Unc80. (A) Schematic representation of the protocol used to achieve differentiation of NG108-15 cells into neuron-like cells. (B) RT-nqPCR with total RNA extracted from differentiated NG108-15 cells, rat adult brain and rat adult liver. The + and − symbols indicates the presence or the absence of the Reverse Transcriptase respectively. The ubiquitously expressed transmembrane Amyloid Precuror Protein (App)-encoding mRNA was used as an internal control. (C) RT-qPCR results with the same RNA samples than in B- showing that both Unc79 and Unc80, but not Nalcn and Nlf1, are expressed in differentiated NG108-15 cells. The gene encoding the RNA helicase Ddx17 was used as a housekeeping gene (n = 4). (D) Representative western blot showing that transfection of the wt NALCN- and its pathogenic variants W1287L, Y578S, L509S, and NLF1-encoding cDNAs in NG108-15 cells results in a high expression level of the corresponding proteins, but not in the eGFP condition.

Back to article page