Figure 4 | Scientific Reports

Figure 4

From: Functional expression of CLIFAHDD and IHPRF pathogenic variants of the NALCN channel in neuronal cells reveals both gain- and loss-of-function properties

Figure 4

Current-voltage (I/V) relationship of the steady-state NALCN current for wild-type (wt) and its CLIFAHDD variant Y578S in differentiated NG108-15 cells. (A) Representative current traces elicited by a membrane voltage step protocol (test pulses from −90 mV to + 30 mV) from a HP at 0 mV, for a mock-transfected cell (top), a NALCN (wt)-transfected cell (middle), a NALCN (Y578S)-transfected cell (bottom). Only the current traces obtained for the test-pulses at −90 mV (in red), −40 mV (in blue), 0 mV (in black) and +20 mV (in orange) are shown. (B) Corresponding I/V relationships for mock transfected cells (black circles, n = 44), NALCN wt (green squares, n = 45) and its pathogenic variant NALCN Y578S (purple triangles, n = 73). Statistical significance was calculated with a Tukey’s multiple comparisons test.

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