Figure 5
Functional activity of hCFTR in nasal and intestinal mucosa. (a) Change in nasal potential difference (NPD) after forskolin stimulation in a chloride-free solution (n ≥ 6 mice/genotype). (b) Short-circuit current (Isc) responses of H+/m− and H−/m− jejuna to forskolin (10 µM)/IBMX (100 µM) stimulation and subsequent treatment with CFTRinh-172 (10 µM), n = 10 and 4 mice, respectively. Only two of four H−/m− mice were treated with CFTRinh-172. *Significantly different from time 0. #Significantly different from time 20 min. (c) Cumulative data for forskolin/IBMX ΔIsc responses of WT (n = 3 mice), H+/m− (n = 10 mice, from Fig. 4D) and H−/m− (n = 3 mice) jejunal sections. a,b,cMeans with the same letters are not significantly different.
