Figure 2
From: LncRNA SAMD12-AS1 promotes cell proliferation and inhibits apoptosis by interacting with NPM1
SAMD12-AS1 promotes cell growth and blocks apoptosis. (a) HepG2 cells were infected with lentivirus carrying SAMD12-AS1 or control virus. Three clones of HepG2 cells stably expressing SAMD12-AS1 (SAMD12-AS1#1, #2 and #3) were chosen, and the amount of SAMD12-AS1 was examined by qRT-PCR. (b) The relative amount of SAMD12 mRNA was examined by qRT-PCR in Ctrl and SAMD12-AS1 cell lines. (c) Growth curves of Ctrl and SAMD12-AS1 cells were determined using CCK-8 assays. (d–f) Ctrl and SAMD12-AS1 cells were injected into 6-week-old BALB/c nude mice (5 per group). Tumor sizes were measured at the indicated times (d). At day 24, the mice were sacrificed, and tumors were dissected out and photographed (e). Tumor weight was measured (f). (g,h) The above cells were treated with etoposide (2 μM) for 48 h. The cell lysates were subjected to caspase-3/7 activity assay (g) and immunoblotting with anti-PARP-1 antibody (h, left). The relative amount of cleaved PARP-1 was quantified using ImageJ (h, right). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. The data in (a–c,g,h) are representative of three independent experiments.
