Figure 5
From: LncRNA SAMD12-AS1 promotes cell proliferation and inhibits apoptosis by interacting with NPM1
SAMD12-AS1 reduces p53 protein via decreasing p53 stability. (a,b) HepG2 Ctrl and SAMD12-AS1 cells were treated with doxorubicin (DOXO) (1 μg/ml) for the indicated times (0, 8 and 12 h) (a, left) and etoposide (4 μM) for 24 h (b, left). Cell lysates were harvested for immunoblotting with the indicated antibodies. The relative amount of p53 was quantified (a,b, right). (c,d) Cells were treated with DOXO (1 μg/ml) for 12 h (c, left) and etoposide (4 μM) for 24 h (d, left). Then, immunoblotting was performed with the indicated antibodies. The relative amount of p53 was quantified (c,d, right). (e,f) HepG2 sh-Ctrl and sh-SAMD12-AS1 cells were treated with doxorubicin (DOXO) (e, left) and etoposide (f, left). Cell lysates were harvested for immunoblotting with the indicated antibodies. The relative amount of p53 was quantified (e,f, right). (g,h) HepG2 Ctrl and SAMD12-AS1 cells (g) or HepG2 sh-Ctrl and sh-SAMD12-AS1 cells (h) were treated with DOXO (1 μg/ml) for 12 h. Total RNA was extracted and subjected to qRT-PCR to quantify p53 mRNA. (i,j) HepG2 Ctrl and SAMD12-AS1 cells (i, left) or HepG2 sh-Ctrl and sh-SAMD12-AS1 cells (j, left) were treated with CHX (25 μg/ml) for the indicated times (0, 20, 40 and 60 min). Cell lysates were harvested for immunoblotting with anti-p53 antibody. The relative amount of p53 was quantified on a log scale (i,j, right). The relative amount of p53 protein was quantified using ImageJ. The results are shown as the means ± S. D. from three independent biological replicates per group. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. The data are representative of three independent experiments.
