Figure 6
From: LncRNA SAMD12-AS1 promotes cell proliferation and inhibits apoptosis by interacting with NPM1
SAMD12-AS1 decreases p53 stability in HepG2-4D14 cells. (a) Total RNA from HepG2-4D14 sh-Ctrl and HepG2-4D14 sh-SAMD12-AS1 cell lines was extracted and subjected to qRT-PCR to quantify the amount of SAMD12-AS1. (b,c) Cells were treated with doxorubicin (DOXO) (1 μg/ml) (b, left) and etoposide (2 μM) (c, left). Cell lysates were harvested for immunoblotting with the indicated antibodies. The relative amount of p53 was quantified (b,c, right). d. HepG2-4D14 sh-Ctrl and HepG2-4D14 sh-SAMD12-AS1 cells were treated with DOXO for 12 h. Total RNA was extracted and subjected to qRT-PCR to quantify p53 mRNA. (e) Cells were treated with CHX (25 μg/ml) for the indicated times (0, 20, 40 and 60 min). Cell lysates were harvested for immunoblotting with anti-p53 antibody. The relative amount of p53 was quantified on a log scale (right). The relative amount of p53 protein was quantified using ImageJ. The results are shown as the means ± S. D. from three independent biological replicates per group. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. The data are representative of three independent experiments.
