Table 2 Kinetic constants of WT GlFBPA and mutants.

From: Structure-based identification of a potential non-catalytic binding site for rational drug design in the fructose 1,6-biphosphate aldolase from Giardia lamblia

Protein

k cat

(seg−1)

Km

(μM)

kcat/Km

(seg−1 M−1)

WT

4.3 ± 0.47

1.8 ± 0.6

2.4 × 106

R259A

0.3 ± 0.04

287.5 ± 61.7

866.2

D278A

2.9 ± 0.32

3.4 ± 0.5

8.5 × 105

R259A/D278A

1.1 ± 0.17

11.2 ± 2.6

9.9 × 104

  1. Values are the mean ± SD for three independent experiments. The previously reported kinetic constants for the WT GlFBPA were kcat 3.55 seg−1, Km 1.7 μM and kcat/Km 2.1 × 106 seg−1 M−1 15.
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