Table 3 Stability parameters of WT GlFBPA and mutants.

From: Structure-based identification of a potential non-catalytic binding site for rational drug design in the fructose 1,6-biphosphate aldolase from Giardia lamblia

Protein

Tm

(°C)

T1/2

(°C)

Cm

(M)

WT

60.9

44.2

1.4

R259A

65.4

56.2

2.5

D278A

47.8

34.4

0.6

R259A/D278A

56.0

42.6

1.1

  1. Tm is the temperature at the denaturation midpoint as indicated by thermal induced denaturation followed by circular dichroism. T1/2 is the temperature that decreases the activity of the enzyme at a half after 20 minutes incubation. Cm is the concentration of urea that reduces 50% of the enzyme activity after incubation by 2 hours at 37 °C. Values are the average of duplicate experiments with S.D. less than 5%.
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