Figure 4
From: Targeting PFKFB3 alleviates cerebral ischemia-reperfusion injury in mice
In vivo AZ67 administration protects mice against neurological impairment, motor discoordination and brain injury caused by a brain ischemia/reperfusion model. (a) Motor coordination, analysed 24 h after a transient MCAO episode in mice, revealed a ~40% performance (rotarod) when compared with the sham-operated animals (100% performance). This effect was significantly prevented by the intravenous administration of AZ67 (60 mg/kg of body weight) immediately after the ischemic episode (~60% performance). (b) Neurological Severity Score (NNS), examined 24 h after a transient MCAO episode in mice, revealed severe neurological deficit according to the Bederson test when compared with the sham-operated animals. This effect was significantly prevented by the intravenous administration of AZ67 (60 mg/kg of body weight) immediately after the ischemic episode. (NNS = 0 both for vehicle or AZ67 in the sham-operated animals). (c) Infarcted brain volume, analysed 24 h after a transient MCAO episode in mice, was ~43% of the brain of the sham-operated animals. This effect was significantly prevented by the intravenous administration of AZ67 (60 mg/kg of body weight) immediately after the ischemic episode (~27% of infarcted volume). Left panel shows pictures of the brain sections of a representative animal for each experimental group. Bar, 1 cm. In all cases, data are mean ± S.E.M. values for 8 male mice. *p < 0.05 (ANOVA followed by the least significant difference multiple range test). See also Supplementary Data 1 and Statistics Table 1.
