Table 4 Primer sequences for chitin synthase 1 (CHS1) and internal control genes used to determine the expression profile in A. gossypii following exposure to buprofezin.

From: Impact of low lethal concentrations of buprofezin on biological traits and expression profile of chitin synthase 1 gene (CHS1) in melon aphid, Aphis gossypii

Primer name

Sequence (5′-3′)

Application

CHS1-F

ATTGCGTCACGATGATCCTT

qRT-PCR

CHS1-R

TGGTCGCTAGACGTTCACAC

qRT-PCR

EF1α-F

GAAGCCTGGTATGGTTGTCGT

qRT-PCR

EF1α-R

GGGTGGGTTGTTCTTTGTG

qRT-PCR

β-Actin-F

GGGAGTCATGGTTGGTATGG

qRT-PCR

β-Actin-R

TCCATATCGTCCCAGTTGGT

qRT-PCR

GAPDH-F

AACAGTTTTTTGAGTGGCGGT

qRT-PCR

GAPDH-R

TGGTGTCAACTTGGATGCGTA

qRT-PCR

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