Figure 2 | Scientific Reports

Figure 2

From: A universal approach to investigate circRNA protein coding function

Figure 2

Formation of circRtn4 with IVS1 and PAT1 introns. (A) Schematic representation of the circRtn4 cassette for pCircRNA-IVS1-Rtn4 and pCircRNA-PAT1-Rtn4 constructs. The IVS1 intron is displayed in red and the PAT1 intron in blue colour. (B) CircRtn4 formation in N2a cells after transfection with circRtn4 constructs. Control, the empty vector control; BE-Rtn4, pCircRNA-BE-Rtn4; DMo-Rtn4, pCircRNA-DMo-Rtn4; IVS1-Rtn4, pCircRNA-IVS1-Rtn4; PAT1-Rtn4, pCircRNA-PAT1-Rtn4; all T-tests were performed in comparison to formation levels of the control sample, ****P ≤ 0.0001, ***P ≤ 0.001, n ≥ 4.

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