Table 2 Comparison of protein levels from various circRNA vectors.

From: A universal approach to investigate circRNA protein coding function

circRNA expressed by different vectors

circRNA formation

co-expression of linear RNA shown in Northern blot

circRNA encoded protein detected by Western blot

ratio to the protein produced by linear mRNA

protein detected by mass spectrometry

References

circLPAR1, circSLC8A1, circCDYL generated by Hansen’s method

yes

yes

no

not applicable

not applicable

13, 54

circRtn4 generated by pCircRNA-BE

yes

not detected

no

not applicable

not applicable

this study36

circRtn4 expressed by pCircRNA-DMo

yes improved

not detected

yes, strong

25%

yes; 76.8% coverage for IP-MS with anti-FLAG antibody

this study36

circ-ZNF609 expressed by circRNA mini vector ZKSCAN1

yes

yes

yes, but weak

0.1%

yes; little coverage for IP-MS with anti-FLAG antibody

21, 49

circMbl expressed by vector with Mbl minigenes

yes

yes

yes

not determined

not determined

50

circE7 expressed by vector with quaking (QKI) protein-binding sites

yes

not determined

yes

not determined

not determined

12, 53

  1. Published circRNAs with protein coding capacity are presented. Not determined: the experiment was not performed or provided in the reference. No data for linear mRNA expression was provided for circMbl and circE7, therefore the rate of protein synthesis from vector-derived circRNA could not be compared. A single peptide mass spectrum from the endogenous circMbl of Drosophila brain was obtained, but no mass spectrometry from protein of the vector-derived circRNA was performed.
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