Figure 2
Devices and tools for automated and parallelized 2D-AEC and 3D-LAC. (a) Overview of our platform with the first liquid handler (used for the application of samples and elution solvents), the disc (carrying four 2D-column arrays and microplates containing 1D-fractions), and the robot for the automated handling of microplates, column arrays, tip racks, elution solvents, and microplate lids. (b–l) some details of chromatographic processing. (b) First liquid handler; 96 1D-fractions are pipetted onto the 2D (AEC)-column array (bi: tips above columns, bii: tip position for discharging). (c) A reservoir containing one elution solvent is transported to the disc. (d) The column array is adjusted to the microplate. (e) Automatic read out of one 384-well microplate containing four 2D-fractions from each of the 96 1D-fractions; (f) Automatic sealing before moving into a refrigerator; (g) Second liquid handler; hit picking and reformatting of fractions; (h) Micro columns for 2D-AEC; (i) Micro columns for 3D-LAC; (j) Parallel 3D (LAC)-chromatography on 24 micro columns; (k) Stacker with two sample plates (96 1D-fractions of two samples); (l) Stacker for tip racks (left) and reservoirs of elution solvents (right).
