Figure 5

Dependence of the degree of PTM (n PTM/PSM) on the concentration of NaCl required for the elution of albumin from AEC matrix. Filled symbols: healthy plasma volunteers and healthy dogs; open symbols: plasma from patients with ESRD and dogs affected by preclinical Alport syndrome. Sub-fractions from plasma pools: orange squares, sub-fractions derived from albumin centric 1D (human plasma: 1D 67, dog plasma: 1D 23); turquoise triangles, sub-fractions derived from LMW albumin (1D 71); blue diamonds, sub-fractions derived from high Mw albumin (1D 63); black circles, sub-fractions from human CSF albumin, centric 1D 23. Data of human plasma were combined from individually pre-fractionated, prepared and analyzed samples from two healthy male volunteers (multi reports from four runs) and from four patients under hemodialysis (multi reports from eight runs). Due to slightly different pooling of the 2D-fractions, data from healthy male and female volunteers were not combined. Results from healthy female volunteers are similar to those from male volunteers and are shown in Supplementary Fig. 8. Accordingly, data from human CSF represent multi reports of nine runs (three sample pools analyzed in triplicate) and data from dog serum are means of technical duplicates from two sample pools. Ordinates: number of affected sites of the modification per PSM (n PTM/PSM) Abscissas: Concentration of NaCl (mM) required for elution of the sub-fraction from the AEC matrix (cf. Fig. 4c). R² is given in the same color as and near to the corresponding data points.