Figure 3
From: Nuclear and cytoplasmic WDR-23 isoforms mediate differential effects on GEN-1 and SKN-1 substrates
Nuclear WDR-23B negatively impacts GEN-1 DNA repair activity. Quantification of the change in GEN-1 protein abundance in response to DNA damage in animals expressing WDR-23A (A; Fig. S3C) or WDR-23B (B; Fig. S3D). Western blot images were quantified using ImageJ after normalization to the actin loading control. n = 3 independent biological replicates for each condition. (C) Experimental outline to measure DNA repair capacity after treatment with 0.01% MMS to induce DNA damage. (D) Animals expressing WDR-23B have decreased viable progeny after DNA damage, which phenocopies gen-1(tm2940) DNA repair mutant animals. n ≥ 8 plates of ≥50 animals for each strain. Data are mean ± s.e.m.; one-way ANOVA with multiple comparisons. *p < 0.05, **p < 0.01. (See Table S1 for more details).
