Figure 3

Loss of galectin-3 affects anti-inflammatory cytokine expression in HT-29 cells. (A) Transcript levels of galectin-3 gene (LGALS3). (B) Galectin-3 protein levels. Transcript levels of (C) TGF-B and (D) IL-10. Data are presented as the mean ± SD (n = 3). *p < 0.05 vs. SCR, **p < 0.01 vs. SCR, ***p < 0.05 vs. SCR, #p < 0.05 vs. LPS, ##p < 0.01 vs. LPS. Veh, treated with phosphate-buffered saline; LPS, treated with lipopolysaccharide; rhGal3, treated with recombinant human galectin-3; SCR, scramble control HT-29 cell; shLGALS3, HT-29 cell stably expressing LGALS3 shRNA.