Table 1 Protein identification by MALDI-TOF/TOF.
Spot no | Protein description | Accession no | Mr(kDa)/pI | Matched peptide sequence | Fold change |
|---|---|---|---|---|---|
404 | 60 kDa heat shock protein, mitochondrial | gi|129379 | 61187/5.70 | IGIEIIKR(41) TVIIEQSWGSPK(79) GYISPYFINTSK(57) VGEVIVTKDDAMLLK(17) ISSIQSIVPALEIANAHR(28) IQEIIEQLDVTTSEYEKEK(37) KPLVIIAEDVDGEALSTLVLNR(95) DMAIATGGAVFGEEGLTLNLEDVQPHDLGK(4) | −7.0 |
503 | Selenium binding protein1 | gi|14290607 | 52928/5.93 | DGLIPLEIR(41) LVLPSLISSR(18) HEIVQTLSLK(87) IYVVDVGSEPR(51) QYDISDPQRPR(30) GGFVLLDGETFEVK(35) VAGGPQMIQLSLDGKR(32) HNVMISTEWAAPNVLR(33) GTWERPGGAAPLGYDFWYQPR(22) DGFNPADVEAGLYGSHLYVWDWQR(38) | −9.0 |
890 | Galectin-3 | gi|2385452 | 26193/8.57 | IALDFQR(35) FNENNRR(35) IQVLVEPDHFK(72) QSVFPFESGKPFK(90) MLITILGTVKPNANR(26) LGISGDIDLTSASYTMI(10) | −5.8 |
950 | Heat shock protein 27 | gi|11036357 | 22826/5.98 | VPFSLLR(52) AQLGGPEAAK(37) DWYPHSR(44) RVPFSLLR(26) QLSSGVSEIR(90) QDEHGYISR(61) HEERQDEHGYISR(46) VSLDVNHFAPDELTVK(108) | 2.2 |
1006 | Transgelin | gi|3123283 | 22653/8.87 | GDPNWFMK(31) QMEQVAQFLK(25) VPENPPSMVFK(64) VPENPPSMVFK(27) GASQAGMTGYGRPR(20) LVNSLYPDGSKPVK(79) TDMFQTVDLFEGK(67) TDMFQTVDLFEGKDMAAVQR(134) TDMFQTVDLFEGKDMAAVQR(43) AAEDYGVIKTDMFQTVDLFEGK(30) | 4.7 |
1014 | Superoxidedismutase | gi|134665 | 24878/8.35 | NVRPDYLK(41) GELLEAIKR(58) DFGSFDKFK(67) GDVTAQIALQPALK.(103) LTAASVGVQGSGWGWLGFNK.(155) HHAAYVNNLNVTEEKYQEALAK(44) | 3.3 |
1188 | Protein S100-A8 | gi|115442 | 10885/6.51 | MGVAAHKK(14) GNFHAVYR(60) GNFHAVYRDDLK(50) ELDINTDGAVNFQEFLILVIK(60) | 9.3 |
