Figure 7
From: Spermatogenesis in haploid males of the jewel wasp Nasonia vitripennis
Nuclear characteristics of trophocytes. (A) Trophocytes (black arrows) adjacent to cystocytes in the testis of a yellow male pupa. (B) High magnification of a trophocyte (top of panel) located adjacent to cystocytes (bottom). Some physical space separates the plasma membranes of these cell types (black arrow). (C) A cyst of germ cells stained for rDNA (green); each cystocyte has a single focus of rDNA. (D) Several trophocytes, each with multiple foci of rDNA, indicating polyploidy. (E) Both trophocytes and cystocytes stained for active RNA polymerase II (red). (F) Ribosomal RNA (green) is expressed in both cystocytes and trophocytes. (G) Transcripts of the germ-line determinant gene, vasa, are expressed only in cystocytes (red). (H) Non-coding transcripts expressed by the B chromosome, PSR, are expressed in trophocytes and not in cystocytes. White arrows in panels (D) through (H) point to trophocytes.
