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Figure 1

From: Screening inducers of neuronal BDNF gene transcription using primary cortical cell cultures from BDNF-luciferase transgenic mice

Figure 1

Construction of a screening assay to examine activity of Bdnf transcription using primary cultures of Bdnf-Luc mouse cortical cells in a 96-well format. (a) Schematic of wild-type Bdnf and Bdnf-Luciferase on a BAC. Detailed information was described previously19,20. (b) Schematic for validating the screening assay system. In 96-well culture plates, Bdnf-Luc mouse (Tg) cortical cells and wild-type (Wt) mouse cortical cells were seeded into wells along lines A–D and lines E–H, respectively. At 13 DIV, cells in wells of columns 2-11 were treated with a high concentration (final 25 mM) of KCl for 6 h. Cells in the wells of columns 1 and 12 were treated with PBS for 6 h. (c) Luciferase activity of each well (left) and the average of luciferase activity (right). Means ± SEM (n = 8 (5 mM KCl), or 40 (25 mM KCl)), ****p < 0.0001 vs. 5 mM KCl (unpaired t-test).

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