Figure 5

Conditioned medium of electrically stimulated ASC induced angiogenesis in vivo. ES CM significantly increased vessel density (A), total vessel network length (B) and total number of branching points (C) compared to CTRL CM in a chorioallantoic membrane (CAM) assay. *p < 0.05; **p < 0.01; ****p < 0.0001. n = 4–7 CAM per group. px = pixel, ns = not significant, NaCl = 0.09% sodium chloride as negative control, nCM = non-conditioned baseline medium, CTRL 72 h = non-stimulated conditioned medium after 72 h of incubation, ES 72 h = electrically stimulated conditioned medium after 72 hours, VEGF = positive control.