Figure 5 | Scientific Reports

Figure 5

From: Cocaine Induces Inflammatory Gut Milieu by Compromising the Mucosal Barrier Integrity and Altering the Gut Microbiota Colonization

Figure 5

Cocaine exposure inhibited trans-epithelial resistance and increased permeability in Caco-2 cells and in mice. Caco-2 epithelial cells were seeded in transwell chambers and exposed to cocaine (10 µM) or left untreated. (A) Exposure of Caco-2 cells to cocaine resulted in decreased trans-epithelial electrical resistance (TEER) and (B) increased paracellular permeability as shown by apical-basal pass of FITC-dextran dye (4 kDa). Results were expressed relative to the initial TEER value and presented as mean ± SEM for three independent experiments. *p < 0.05. (C) Mice were administered cocaine (20 mg/kg, i.p.) or saline for seven days and orally gavaged with FITC Dextran (4 kDa) on the final day of cocaine injection followed by collection of blood 4 h post FITC dextran gavage. (n = 9/group), p = 0.08.

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