Figure 4 | Scientific Reports

Figure 4

From: The regulation of the sulfur amino acid biosynthetic pathway in Cryptococcus neoformans: the relationship of Cys3, Calcineurin, and Gpp2 phosphatases

Figure 4

(a) Growth evaluation of wild type (H99), cys3 mutants (CNU123 and CNU124), and complemented strain (CNU136) by spot dilutions on YEPD added with 20 mM cysteine in different pHs (6.4 and 7.0 and 7.5); (b) virulence assay in G. mellonella with H99 as positive control, PBS as negative and test strains cys3Δ (CNU123), and complemented strain (CNU136). Virulence assay expressed as percentage of larvae survival during 10 days at 30 °C. There are statistical significant differences among the survival curves at a p < 0,001 (***).

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