Figure 5 | Scientific Reports

Figure 5

From: The regulation of the sulfur amino acid biosynthetic pathway in Cryptococcus neoformans: the relationship of Cys3, Calcineurin, and Gpp2 phosphatases

Figure 5

GFP-Cys3 cellular localization and expression pattern in western blotting. (a) Percentage of GFP and DAPI localization accessed by fluorescence microscopy in CNU080 submitted to various nutritional conditions (n > 100 cell/treatment, asterisks represent the significant differences (*p < 0.01 and ***p < 0.001). (b) Fluorescence microscopy images showing localization of GFP and DAPI in YEPD (top) and SD-N + Cys/Met (Bottom). Merge is the overlay of DAPI and GFP images and localization is represented by the yellowish color; (c) western blotting of proteins extracted from strain CNU080 cultivated for 1 and 2 h in YEPD and SD-N + Cys/Met. Mouse Anti-GFP primary antibody was used at 1:2000 dilution and secondary anti-mouse horseradish peroxidase-linked antibody was applied at 1:2000 dilution. Loading control was done by detecting histone H3 protein with rabbit anti-His3 antibody (1:2000) as primary and anti-rabbit secondary antibody horseradish peroxidase linked (1:2000). The panel is a crop of a bigger image. The original images are shown in Supplementary data.

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