Table 1 Proteins identified by LC/MS and MALDI/TOF after IP of GFP-Cys3. CNU080 cell extracts were collected in two growth conditions (2 h in YEPD and SD).

From: The regulation of the sulfur amino acid biosynthetic pathway in Cryptococcus neoformans: the relationship of Cys3, Calcineurin, and Gpp2 phosphatases

Immunoprecipitation*

Amino acid sequence identified

% coverage

YEPD

Sulfate adenylytransferase (MET3)

14.80

Elongation fator 1-alpha (TEF1)

32.13

Glyceraldehyde-3-phosphate dehydrogenase (GPD)

22.12

Cytochrome c peroxidase_mitochondrial (CCP1)

33.48

40 S ribossomal protein S0 (RPS0)

31.33

40 S ribossomal protein S1 (RPS1)

32.24

ATP-dependent RNA helicase eIF4A (TIF1)

13.10

ATP-dependent RNA helicase SUB2 (SUB2)

11.90

Mitochondrial import inner membrane translocase subunit (TM8)

26.14

SD

Protein Phosphatase (GPP2)

11.44

Serine/threonine protein phosphatase 2B catalytic subunit A1 (CNA1)

9.23

Calcineurin subunit B1 (CNB1)

10.10

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