Figure 5
From: An automated pipeline for the screening of diverse monoterpene synthase libraries
Relative product profiles obtained for each active variant using VAR3-PinS as template. A comparison of the relative titres of the 9 common monoterpene products found in native and VAR3-PinS is shown. A darker shade of green indicates a greater relative accumulation of that compound. Variants with mutations in region 1 are in blue, region 2 in red, and region 3 in purple. Data for native (WT) and VAR3-PinS was obtained from Leferink et al.8. Other monoterpenoid products detected are β-phellandrene (for variants C335A, C335G, A557S and M559V) and sabinene-hydrate (for variants C335A, C335G, and A560C). Other sesquiterpenoid products detected are farnesene (for variants M559V and Q561F), nerolidol (for variant M559V), and farnesol (for variant G444C). A full breakdown of the product profiles can be found in Table S6 in the Supplementary Information.
