Figure 2
Antimalarial drug treatments cause minor changes to the diversity indices of the gut microbiota in mice treated with AA. Alpha diversity of the gut microbiota as measured by observed OTUs in mice treated with (A) saline water and (B) artesunate and amodiaquine. (C) Bray-Curtis beta diversity for samples treated with either water or artesunate and amodiaquine. The distances compared are between the pooled day 0 samples and each treatment group at each time point followed by comparisons between treatments at each time point. (D) Weighted UniFrac diversity for samples treated with either saline or artesunate and amodiaquine. (E) Unweighted UniFrac diversity for samples treated with either saline or artesunate and amodiaquine. Data (mean±SEM) in A-B were analyzed by one-way ANOVA with Tukey’s multiple comparisons test but showed no significant values (p > 0.05); data in (C–E) were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test followed by a two-tailed t test for treatment comparisons at each time point. * = comparison between same-day treatments; # = comparison between treatment day and day 0 p.t. 1 symbol = p < 0.05; 2 symbols = p < 0.01; 3 symbols = p < 0.001; 4 symbols = p < 0.0001.
