Figure 4
From: Lyssavirus matrix protein cooperates with phosphoprotein to modulate the Jak-Stat pathway
Mutated P and/or M proteins attenuate RABV virulence correlating with differing innate immune-response activation. Three-week-old BALB/c mice (6 per experiment) were infected intramuscularly with 1000 FFUs of Tha, Th2P, Th4M or Th2P-4M RABVs and monitored for 21 days. At the end of the experiment, 3 mice survived when infected with Th2P-4M, named Th2P-4M survivors. (A) The mice were sacrificed day 9 post-infection (denoted D9) or when late infection symptoms appeared (endpoint). mRNA, extracted from their brains, was subjected to RT–qPCR to analyze expression of the indicated genes, normalized to the GAPDH-reporter gene level in Mock-infected mice. RABV mRNA (B), and ISG15 (C), MxA (D), IFNβ1 (E), Tyk2 (F), Jak1 (G) and Stat1 (H) gene expression was measured. Results show means ± standard deviation (T-bars) expressed in arbitrary units (AU). (NA): non applicable; *p < 0.05 compared to Mock-infected D9 samples and #p < 0.05 compared to Mock-infected End-point samples. Th2P-4Ms*: Th2P-4M survivors.
