Figure 5
Blocking α4 integrin improves cognitive functions in 3xTg-AD mice. Groups of 3xTg-AD mice were treated with a blocking antibody specific for α4 integrin (Anti-α4 integrin) or an isotype control antibody (Isotype) for 4 weeks starting at 6 months of age. Wild-type age-matched control mice were treated with endotoxin-free PBS. (a) Results showing the percentage of alternation in the Y-maze test (left) and the percentage of freezing in the contextual fear-conditioning (CFC) test (right) Y-maze, n = 11 mice (5 F, 6 M) for the WT ctrl group, n = 9 mice (4 F, 5 M) for the isotype group, and n = 8 mice (4 F, 4 M) for the anti-α4 integrin group. For CFC, n = 14 mice (7 F, 7 M) for the WT ctrl group, n = 11 mice (5 F, 6 M) for the isotype group, and n = 11 mice (6 F, 5 M) for the anti-α4 integrin group (*P < 0.05, **P < 0.01; Mann-Whitney U-test). (b,c) Morris water maze test. (b) The escape latency to reach the hidden platform during training period (two-way ANOVA between groups: **P < 0 01 and between training days; **P < 0.01; Multiple t tests within the same day *P < 0.05 wild-type control (WT ctrl) versus isotype control; #P < 0.05 isotype control versus treatment group). (c) Number of platform location crossings and latency to cross platform location (*P < 0.05, **P < 0.01; Mann-Whitney U-test). Values represent mean ± SD of the data obtained from a representative experiment with n = 11 mice (5 F, 6 M) for the WT ctrl group, n = 8 mice (4 F, 4 M) for the isotype group, and n = 10 mice (5 F, 5 M) for the anti-α4 integrin group.
