Figure 3
From: Exhaustion of CD4+ T-cells mediated by the Kynurenine Pathway in Melanoma
Functional consequences of IDO1 blockade on CD4+ T cell- MCLs co-culture. (a) The proliferation of CD4+ T-cells in the presence of MLCs alone and with the IDO1 blockade (b) KYN production by HPLC after 48 h and (c) Ratio of CTLA4+, FOXP3+ or PD-1+ population among CD4+ T-cells by flow cytometry (d), CTLA4, FOXP3, PD-1 IL-10, IL-6, TGFβ and TNFα mRNA expression after five days were analysed by real-time PCR and normalized to RPL13A in CD4+ T-cells. IDO1i: IDO1 inhibitor (INCB024360). Graphs show individual data, and horizontal lines show mean ± s.e.m. *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005 by One-Way ANOVA (non-parametric) and independent samples t-test (two-sided) and the Tukey multiple comparison procedure was used to determine significance; and adjusted P values for the differences in group means can be seen in Table S6, n > three biological replicates cultured with four different melanoma cell lines.
