Figure 2

Phylogenetic relationship of different classes of plant subtilases. The tree was constructed with IQ-TREE by employing the Maximum Likelihood method (model: WAG + R8, predicted by Modelfinder). The circles around the phylogenetic tree (from center to periphery) represent SBT1-5, and the respective species distribution, indicated by different colors. Some bacterial subtilase genes from the phylogenetic tree of the S8A subfamily (Supplementary Fig. S1) were selected as outgroup. Histograms of two outer rings are numbers (red histogram) and average length of introns (green histogram) respectively. For each clade, numbers above branches indicate bootstrap values based on 200 replications.