Table 1 ATPase activity of wild-type KaiC and its variants at different pressures.

From: Pressure accelerates the circadian clock of cyanobacteria

Pressure/bar

kcat ± stdeva/day−1 (n)b

KaiC-WT

CI domain (CI-ATPase)

KaiC E77QE78Q (CII-ATPase)

KaiC R393C

KaiC F470Y

1

14 ± 2 (17)

11 ± 2 (13)

2.3 ± 0.6 (11)

20 ± 3 (5)

21 ± 3 (4)

100

18 ± 4 (7)

12.0 ± 0.8 (5)

4 ± 1 (6)

n.d.

n.d.

200

22 ± 3 (10)

13 ± 1 (7)

5 ± 2 (6)

23 ± 2 (5)

25 ± 2 (4)

Ratio (200 bar/1 bar)

1.6

1.2

2

1.1

1.2

  1. aStandard deviations. bNumber of protein samples. Concentration of each protein sample was separately adjusted, irrespective of the sample lot.
Back to article page