Figure 4 | Scientific Reports

Figure 4

From: Formation of the Francisella tularensis Biofilm is Affected by Cell Surface Glycosylation, Growth Medium, and a Glucan Exopolysaccharide

Figure 4The alternative text for this image may have been generated using AI.

Electrophoretic protein profiles of LVS variants or mutants grown as a biofilm or are planktonic cells from the biofilm broth supernatant. Bacterial strains were grown as a biofilm in CDMB for 25 days, the medium above the biofilm was removed, and the planktonic cells harvested by centrifugation. Planktonic cells in the medium and cells in the biofilm were extracted with 1 M urea. The protein profiles were resolved by SDS-PAGE on 4-12% gels and stained with silver. Strains: L, LVS; P, LVS_P10; LM, LVSΔ1423-22; WM, WbtIG191VΔ1423-22; M, Molecular size standards. The figure is a compilation of four separate gels containing samples prepared during the same experiment (left two are from planktonic phase cells and right two are from biofilm cells), which have been cropped to present the most relevant information (original shown in Supplementary Fig. S3). Precipitates of the broth medium are not shown. Lanes of WbtIG191V (W) have been deleted from the planktonic cells (far left) and from the biofilm cells (far right) due to lack of differences with WbtIG191VΔ1423-22 (WM). Repeated subculture in CDMB had little effect on protein composition, but loss of surface glycoses, particularly O-Ag, or growth as a biofilm substantially altered the protein profile.

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