Figure 3
From: Connective tissue growth factor is correlated with peritoneal lymphangiogenesis
Positive correlation between the increase in connective tissue growth factor (CTGF) and the increase in vascular endothelial growth factor-C (VEGF-C) in human peritoneal mesothelial cells (HPMCs) treated with transforming growth factor-β1 (TGF-β1). HPMCs were collected from the spent peritoneal dialysis (PD) effluents derived from 21 PD patients with various peritoneal transport functions. Nine patients showed lower peritoneal permeability (dialysate to plasma ratio of creatinine, D/P Cr < 0.64), and the other 12 patients showed higher peritoneal permeability (D/P Cr > 0.65). Cultured HPMCs were treated with 5 ng/ml recombinant human TGF-β1 for 12 h and harvested. Increases in CTGF and VEGF-C messenger RNA (mRNA) following incubation with TGF-β1 were analyzed with quantitative polymerase chain reaction. A positive correlation was observed between CTGF and VEGF-C mRNA amplification in HPMCs treated with TGF-β1. 18 S ribosomal RNA (rRNA) was used as an internal reference. Pearson correlation was used for the analysis.
