Figure 6
From: Connective tissue growth factor is correlated with peritoneal lymphangiogenesis
Small interfering RNA (siRNA)-mediated knockdown of connective tissue growth factor (CTGF) reduced vascular endothelial growth factor-C (VEGF-C) upregulation in human peritoneal mesothelial cells (HPMCs) treated with transforming growth factor-β1 (TGF-β1). HPMCs were collected from the peritoneal dialysis (PD) effluent derived from a PD patient with high peritoneal permeability. CTGF messenger RNA (mRNA) expression was inhibited by siRNA in cultured HPMCs treated with 5 ng/ml recombinant human TGF-β1 for 24 h. Quantitative polymerase chain reaction analysis showed that the TGF-β1-induced mRNA upregulation of both CTGF and VEGF-C was reduced by CTGF siRNA compared with control siRNA. 18 S ribosomal RNA (rRNA) was used as an internal reference. Graphs show means ± SD (n = 3 for each group).
