Figure 2 | Scientific Reports

Figure 2

From: Analyses of virus/viroid communities in nectarine trees by next-generation sequencing and insight into viral synergisms implication in host disease symptoms

Figure 2

Confirmation of the identified viruses and viroids by RT-PCR. RT-PCR was used to amplify partial sequences of (A) APV1, (B) APV2, (C) APV3, (D) ACLSV, (E) CGRMV, (F) CNRMV, (G) GRGV, (H) PBNSPaV, (I) PeVD, (J) NSPaV, (K) PaLV, and (L) PLMVd using virus or viroid-specific primer pairs from nectarine tree RNA samples T01, T02, T03, T04, and T05. Amplified DNA fragments were examined by electrophoresis on 1.5% agarose gels and stained with EtBr. M, DNA size 2000 marker; N, negative control.

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