Table 3 Oligonucleotide primers used for conventional, nested-PCR and real-time PCR of ssrA, rpoB, groEL genes and ITS region.
From: Prevalence and diversity of Bartonella species in small rodents from coastal and continental areas
Primer | Locus | Nucleotide sequence | Reference |
|---|---|---|---|
ssrA-R1 | ssrA | AAGGCTTCTGTTGCCAGGYG | This study |
ssrA-F1 | AGTTGCAAATGACAACTATGCGG | ||
ssrA-P1a | FAM-ACCCCGCTTAA ACCTGCGACGGTT | ||
RpoB-F | rpoB | CGCATTGGYTTRCTTCGTATG | Renesto et al.24 |
RpoB-R | GTRGAYTGATTRGAACGYTG | ||
BTNgroEL1 | groEL | GAAGATGTGGAAGGTGAA | |
BTNgroEL2 | TCACGGTCATAGTCAGAAG | ||
WITS-Fb | ITS | ACCTCCTTTCTAAGGATGAT | |
WITS-Rb | CTCTTTCTTCAGATGATGATCC | ||
Bh311-332Fc | CTCTTTCTTCAGATGATGATCC | ||
Bh473-452Rc | AACCAACTGAGCTACAAGCCCT | ||
Inner ITS-R (ITS)c | GCGGTTAAGCTTCCAATCATA |
