Figure 4
From: Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors
Metabolite concentrations and process parameter profiles of hiPSC suspension cultures in bioreactors and static well-plate suspension control cultures. In situ imaging facilitated the evaluation of aggregate concentrations on in-flow images (A). At-line analysis of culture medium supernatant allowed for metabolite concentration assessment of (B) glucose, (C) lactose, (D) glutamine, (E) ammonia, (F) and lactate dehydrogenase activity. Bioreactors were equipped with optical sensor spots for measuring physical process parameters (G) pH and (H) dissolved oxygen. Error bars indicate mean ± SD, n = 3.
