Figure 6
From: RNA modulates aggregation of the recombinant mammalian prion protein by direct interaction
CD spectra of rPrP (5 μM) aggregated in the presence of different RNAs (A) domain V rRNA, (B) DHFR mRNA, (C) GFP+ mRNA, at 1:0.05 protein to RNA ratio. For each sample, three spectra were collected; protein alone (red trace); RNA alone (green); rPrP-RNA complex (blue trace). The sum of the protein alone and RNA alone spectra produced the cumulative spectra (yellow trace); RNA spectra subtracted from the complex spectra (blue trace) generated the difference spectra (dotted line).
