Figure 3

The effect of increased DOX concentration on the viable THP-1 cells (a) shows a consistent shift from control cells. The shift is more dominated along PC2, which is related to changes in lipid and nucleic acid content and is supported by the reconstructed spectra (b). DOX also influences the non-viable cells, with the influence being more significant at high DOX concentrations (c). The scores transit along PC1, which is related to nucleic acid and protein content. The reconstructed spectra also show variations in several band positions (d) accordingly. The solid black lines in (a,c) are the decision boundary in between the viable and non-viable cells obtained from the PCA-SVM model.