Figure 4

PDAC associated genes mainly fall into the V-ex and W-ex groups. (a) The gene mutation frequency of all V-ex, W-ex and N-ex mutations. (b) Comparison of the DNA allele frequency and RNA allele frequency for the most well-known PDAC mutations (KRAS, CDKN2A, SMAD4, TP53). (c) Fraction of mutations that are associated with the Cosmic and Tamborero cancer gene datasets for each mutation expression group. Statistical significance was performed using a two-proportion z-test between each of the mutation expression groups. (d) A network generated from the V-ex, W-ex, and N-ex mutated genes that were present in either the Cosmic or Tamborero datasets. (e) The distribution of mutation expression groups from the top 50 ranked mutated genes outputted by the driver mutation detection methods 2020+, Muffin, and OncodriveFM. (f) A Fisher’s exact test was performed on three driver mutation detection methods to quantify their ability to identify cancer associated genes from the Cosmic and Tamborero, based on their 50 top-ranked mutated genes. Cancer gene statistical significance was calculated for either all top 50 mutated genes or the top 50 mutated genes that did not contain an N-ex mutation.