Table 5 List of primers used in both PCR and qPCR analysis.
Sl. No. | Gene Name | Forward Primer | Reverse Primer | Product Length |
|---|---|---|---|---|
PCR Primers | ||||
1 | csf1r | TGGTGRTCACGGAGTACTGTY | ATGTGCTGGAACCTSGAMSC | 671 |
2 | SR-B1 | TGATCAAYGGMACAGCSGGGG | GTTTGMAGAGAGCGGCTACA | 466 |
3 | StAR5 | ATTACATAMASACKGCCAGATCK | AGAGGCTTCAAYCAYCCRTG | 411 |
4 | GSTA2 | AGCARGTGCCTTTGGTKGAAA | GAGCCGTGCTGAYGTTCAYY | 274 |
5 | PLIN2 | TGAAGTCTGTSTGYGAGGTGG | CTTCAGASCACATGTGTGGT | 806 |
6 | StAR3/MLN64 | CGTYCGAYCTKCTYTTCATCA | GAGAGTGTATCTGGCMGCAG | 349 |
7 | BCDO2 | GCTTTGATGCAYCGCTTTCA | AGTGTTCATYGAGCAGCCCA | 534 |
qPCR Primers | ||||
1 | csf1r | GAGTGGCCAAAATCTGCGAC | GGCATTCTGCTGTGGGAGAT | 175 |
2 | SR-B1 | GTCGGGTCTTCTGAACGTGG | GACATACACCCGGAGACAGG | 162 |
3 | StAR5 | GAGTTCGCAGGATGCGTCTA | CAGAACCGTCACCCCTTCAG | 144 |
4 | GSTA2 | AGCTTGTGCAGACAAAGGCTA | ACCACCTGACAACAAGCAGAA | 166 |
5 | PLIN2 | AACGTTCACCTCGGTTGCTT | TGCTAAAGAGGCAGTGACAGG | 177 |
6 | StAR3/MLN64 | GTTTTGGCCGTGTTCAGGTT | TACGTGCTTCCCATCACCTC | 176 |
7 | BCDO2 | TGACGTACCGGAGTCGTTTTT | TTCAGCGTTTCCTGACCAGA | 137 |
