Figure 1 | Scientific Reports

Figure 1

From: Environmental DNA size sorting and degradation experiment indicates the state of Daphnia magna mitochondrial and nuclear eDNA is subcellular

Figure 1

Overview of the study design from sampling to DNA quantification and qPCR amplification and analysis. The four tanks (mesocosm) used in the experiment (3 treatments plus 1 control) are depicted at the top of the figure. The subsequent workflow is depicted following the arrows downwards whereby 500 ml samples were collected from each tank at days 0, 1, 3, 6, 17 and 30, which were sequentially filtered through three filter sizes 10, 1 and 0.2 μm with the final filter effluent water retained and stored as depicted with a conical tube. All four samples sizes (3 filters and effluent water) were extracted separately and each extract was quantified for total DNA using a Qubit fluorometer and quantified using qPCR using Daphnia magna specific primers targeting the 18S and COI gene regions.

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