Figure 3
Decreased thymocytes in OgaVav-Cre mice. (a) Quantitation of total number of thymocytes from the indicated genotype. (b) Representative flow cytometric analysis, with boxes depicting gating of thymus cells including early thymic progenitors (ETP, Kit+CD25−) DN2/3 (CD25+), Early ETPs (Kit+CD25−Flt3+), DN2a (CD25+Kit+CD44+), DN2b (CD25+KitintCD44+), DN3 (CD25+Kit−CD44−) and analysis of double positive (DP) CD8+ and CD4+ T cells. (c) Graphs depicting the absolute numbers of the indicated cell populations for the indicated genotype. Black bars represent wildtype and blue bars represent OgaVav-Cre. N = 4–6, error bars represent standard deviation, *p < 0.05, **p < 0.01, ***P < 0.001, ****P < 0.0001 as determined by t-test.
