Figure 2

TLR stimulation induces PLK2 and 3 mRNA expression. Whole blood was treated with R848 or LPS at the indicated concentrations and for 2 or 6 h. Subsequently, total RNA was isolated, genomic DNA digested, reverse-transcribed and used in quantitative real-time PCR for the indicated PLKs relative to the TBP housekeeping gene. Four donors were assayed in triplicates. Biological means +/− SEM are shown.