Figure 1 | Scientific Reports

Figure 1

From: Proteasome dysfunction in alveolar type 2 epithelial cells is associated with acute respiratory distress syndrome

Figure 1

Targeted deletion of RPT3 in AT2 cells is lethal. (a) SftpcWT/CreER:RPT3F/F (RPT3F/F) and SftpcWT/CreER (Cre) mice were fed tamoxifen chow for 7 days to promote recombination at the Psmc4 (RPT3) locus in AT2 cells, then switched to regular chow on day 7. The efficiency of recombination was assessed in AT2 cells (isolated on day 7) by quantitative PCR (b) and Western blotting (c). (d) Densitometry of Western blot in c. **p < 0.01, ****p < 0.0001 by one-way ANOVA with Tukey’s multiple comparison test. RQ: relative quantitation. (e). Kaplan Meier survival curve. ****p < 0.0001 by Mantel-Cox test. Full-length blots are presented in Supplementary Fig. 9.

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