Figure 5
RPT3 deficiency results in increased cell stress and death. (a) Heatmap of differentially expressed cell stress response genes in AT2 cells isolated on D9. (b) Western blot analysis of cell stress markers in 30 μg of AT2 cell lysates separated by SDS-PAGE. (c) Heatmap of differentially expressed apoptosis-associated genes in AT2 cells isolated on D9. (d) Percentage of TUNEL+ AT2 cells assessed by morphometric analysis of Fig. S6a. Control included both RPT3F/F mice (SftpcWT/CreER:RPT3F/F mice without tamoxifen treatment) and Cre mice that were fed tamoxifen chow for 7 days. Heatmaps were z-score normalized. Adjusted p-value < 0.05. n = 3 mice/genotype. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with Tukey’s multiple comparison test. Full-length blots are presented in Supplementary Fig. 9.
