Figure 10 | Scientific Reports

Figure 10

From: Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence mutation detection in circulating tumour DNA

Figure 10

Optimisation of ddPCR cycling conditions. Increasing extension time and reducing ramp rate from (a) standard results in clouds which are (b) more compact with less rain. (c) Rain can be reduced further by increasing the number of PCR cycles from 40 to 50. Note that altering cycling conditions can change the amplitude of some clouds. Increasing the cycle number up to 50 can lead to the emergence of non-specific double-positive droplets (arrow).

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