Figure 3 | Scientific Reports

Figure 3

From: Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence mutation detection in circulating tumour DNA

Figure 3

ddPCR assay optimisation workflow. The workflow highlights the key stages in optimisation of a sensitive, specific and reproducible ddPCR assay for (a) singleplex and (b) multiplex assays. Steps in (a) must be completed before (b) multiplex assay optimisation workflow commences. For an explanation of the purpose of each stage, refer to Supplementary Tables 2 and 3.

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